
The path name column is optional if all image files are in the base folder, this column is not needed. "Image_PathName_CY3" would supply the path names for the CY3-stained images. For instance, "Image_FileName_CY3" would supply the file name for the CY3-stained image, and choosing the Load images based on this data? option allows the CY3 images to be selected later in the pipeline. The image's name within CellProfiler appears afterward. Columns whose name begins with Image_FileName or Image_PathName: A column whose name begins with "Image_FileName" or "Image_PathName" can be used to supply the file name and path name (relative to the base folder) of an image that you want to load.This is a convenient way for you to add data from your own sources to the files exported by CellProfiler. Columns with any name: Any data loaded via LoadData will be exported as a per-image measurement along with CellProfiler-calculated data.Below, we describe how the column nomenclature allows for special functionality for some downstream modules:

There are many reasons why you might want to prepare a CSV file and load it via LoadData. Subsequent rows provide the values for each image cycle. The fields in this row provide the labels for each column of data. The LoadData module uses the first row of the file as a header. Text values may be optionally enclosed by double quotes. The lines of the file represent the rows, and each field in a row is separated by a comma. These files can be produced by saving a spreadsheet from Excel as "Windows Comma Separated Values" file format. The module currently reads files in CSV (comma-separated values) format. However, old pipelines loaded into CellProfiler that contain this module will provide the option of preserving them these pipelines will operate exactly as before. This module loads a file that supplies text or numerical data associated with the images to be processed, e.g., sample names, plate names, well identifiers, or even a list of image filenames to be processed in the analysis run.ĭisclaimer: Please note that the Input modues (i.e., Images, Metadata, NamesAndTypes and Groups) largely supercedes this module. Load Data loads text or numerical data to be associated with images, and can also load images specified by file names.
